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Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology

DOI: 10.1016/j.ultsonch.2011.05.004
Title: Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology
Journal Title: Ultrasonics Sonochemistry
Volume: 18
Issue: 6
Publication Date: November 2011
Start Page: 1279
End Page: 1286
Published online: online 8 May 2011
ISSN: 1350-4177
Affiliations:
  • Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran
  • Abstract: ic technology was applied for extraction of polyphenols and antioxidants from the rice bran using ethanol as a food grade solvent. Response surface methodology (RSM) was used to optimize experimental conditions for extraction of polyphenols and antioxidants. Three independent variables such as solvent percentage (%), temperature (°C) and time (min) were studied. Effect of ethanol concentration was found to be significant on all responses. Total phenolic content (TPC) varied from 2.37 to 6.35 mg gallic acid equivalent/g of dry sample. Antioxidant activity of the extracts was determined by the ferric reducing antioxidant power (FRAP) assay and scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. FRAP and DPPH values varied from 31.74 to 57.23 μmol Fe2+/g of dry sample and 16.88% to 55.61% inhibition, respectively. Extraction yields ranged from 11 to 20.2%. Optimal ultrasonic-assisted extraction (UAE) conditions were identified as 65–67% ethanol, 51–54 °C, 40–45 min. The experimental values agreed with those predicted by SRM models, thus indicating suitability of the model employed and the success of RSM in optimizing the extraction conditions.
    Accepted: 4 May 2011
    Received: 29 December 2010
    Revised: 2 May 2011
    Corresponding information:
    Fax: +98 841 2227022
    Email: r.tabaraki@ilam.ac.ir rezatabaraki@yahoo.com

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